Journal: Scientific Reports

Article Title: Bacterial bioburden and community structure of potable water used in the International Space Station

doi: 10.1038/s41598-022-19320-3

Figure Lengend Snippet: Bacterial community structure in the PWD water determined by 16S rRNA gene amplicons sequenced on Illumina MiSeq, iSeq, or Nanopore MinION.

Article Snippet: A 16S rRNA sequencing library was constructed from 10 µL of extracted DNA using the 16S barcoding kit (Oxford Nanopore Technologies).

Techniques:

Journal: Scientific Reports

Article Title: Bacterial bioburden and community structure of potable water used in the International Space Station

doi: 10.1038/s41598-022-19320-3

Figure Lengend Snippet: Relative abundance of bacterial species in the PWD water determined by 16S rRNA gene targeted amplicon sequencing with the MinION sequencer. Genus and species with an abundance >1% are presented.

Article Snippet: A 16S rRNA sequencing library was constructed from 10 µL of extracted DNA using the 16S barcoding kit (Oxford Nanopore Technologies).

Techniques: Amplification, Sequencing

Journal: Cell reports

Article Title: Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

doi: 10.1016/j.celrep.2020.108222

Figure Lengend Snippet: (A) Illustration depicting procedures for acquiring the transcriptome and timestamp barcode information from each single cell. ESCs are engineered to express an inducible Cre (Cre-ERT2-T2A-BFP), a Cre reporter (lox-RFP-STOP-lox-GFP), and a timestamp barcoding system containing a static barcode (N 10 nucleotides) and an inducible tandem-loxP barcode (E2-crimson-tandem-loxp-UCI). ESCs are sorted into culture plates based on reporter expression (RFP, BFP, and E2-crimson) (as in ). Recombination of the tandem-loxP sequence is induced by addition of Cre-ERT2. At the end of the 14-day time course, cells are harvested. The timestamp barcode is amplified using targeted primers and sequenced using Oxford Nanopore long-read sequencing. The transcriptome is profiled as before . (B) The black histogram shows barcode distribution calculated from NGS data. The red cumulative frequency plot on the same plot shows that that 95% of barcodes are detected with 50 sequencing reads per UCI, consistent with observations from other barcoding studies . (C) Barplot showing the length distribution of long sequencing reads after Cre-ERT2 induction. The tandem-loxP sequence contains 5 converging pairs of loxP sites with 9 spacer sequences, making an intact total of 2,317 bp. The full recombined product yields a 621-bp fragment. (D) Pie charts displaying the number of unique loxp recombined barcodes (left) and the number of unique loxP recombined barcodes+UCI (right). 5,000 static UCIs and a total of 155 achievable, temporally controlled tandem-loxP barcodes yield 514 unique barcodes in EBs after applying strict filtering criteria ( – ; ). See also and and .

Article Snippet: 1D Native barcoding kit , Oxford Nanopore Technologies , EXP-NBD104, EXP-NBD114.

Techniques: Expressing, Sequencing, Amplification

Journal: Cell reports

Article Title: Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage Decisions in Developing Embryoid Bodies

doi: 10.1016/j.celrep.2020.108222

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: 1D Native barcoding kit , Oxford Nanopore Technologies , EXP-NBD104, EXP-NBD114.

Techniques: Recombinant, Staining, Ligation, Sequencing, Software